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[中文摘要]
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针对鞭毛虫样品制备中的难点,以Bodomorphaminima ,Hollande 1942为材料,对一类个体微小的异养性鞭毛虫的扫描电镜样品的制备做了初步探讨。以载玻片为载体,0 .1%的多聚赖氨酸为粘附剂,2 %的戊二醛为固定剂,采用临界点法干燥,获得了较为满意的扫描电镜照片。该技术可简化样品制备步骤,减少样品损失,对于其他原生动物样品和游离细胞样品扫描电镜的制备也有积极的借鉴意义。
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[外文摘要]
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The preparation of
flagellate specimens for surface scanning electron microscopy (SEM) was
performed using Bodomopha minima, Hollande 1942 as the experimental material. Satisfying
SEM micrographs were obtained using glass slides as carriers, 0.1%
poly-L-Lysine as the bond, 2% Glutaraldehyde as
the fixing solution, and the critical point drying method. This preparation
will prove to be useful for other protozoa and dissociative
cell specimens.
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