生物化工专题数据库-----海洋生物

 

[篇名]

盐藻多糖提取工艺的研究

[著者]

韩玉谦; 隋晓

[机构]

中国海洋大学国家海洋药物工程技术研究中心,山东 青岛 266101

[中文关键词]

盐藻多糖; 正交设计; 提取工艺; 酶解

[外文关键词]

\ Polysaccharides from D.Salina; orthogonal experimental design; extraction process; enzymatic hydrolysis

[分类号]

Q946.3Q556

[刊名]

中国海洋大学学报

[出版时间]

20056

[来源]

 VOL35,NO.6._991—993

[中文摘要]

探讨盐藻D.peircei多糖的提取工艺。采用正交实验确定了提取盐藻粗多糖的最佳工艺条件,通过酶解方法脱除其蛋白。实验结果表明,提取盐藻粗多糖的最佳工艺条件为100,提取10h,固液比116,pH=9,通过中性蛋白酶对其酶解,可脱除其部分蛋白,使糖含量达到29.01%,初步确定此多糖为含有硫酸基和己糖醛酸的蛋白多糖。

[外文摘要]

The extraction process of polysaccharides from D.salina was studied by the orthogonal experimental design.The protein was removed by enzymatic hydrolysis.The Results showed that the optimum extraction condition was to add 16 times in quantity of water to the D.peircei,and add 40% NaOH to the mixture until pH=9,and extracting at 100 for 10 hours.The polysaccharides from D.salina.was deproteinated by neutral protease.The content of the polysaccharides was 29.01%.The polysaccharides from D.Salina.were sulfate...