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[篇名]
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[著者]
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孙燕; 吕志华
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[机构]
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中国海洋大学海洋药物教育部重点实验室，山东 青岛 266003
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[中文关键词]
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褐藻胶寡糖; 酶解; 高效毛细管电泳
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[外文关键词]
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alginate oligomers; enzymatic hydrolysis; High Performance
Capillary Electrophoresis（HPCE）
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[分类号]
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Q178.53；O652
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[刊名]
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中国海洋大学学报
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[出版时间]
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2005年6月
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[来源]
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VOL35,NO.6._984—990
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[中文摘要]
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采用高效毛细管电泳法(HPCE)对酶解褐藻胶寡糖进行了分离分析,考察了缓冲液浓度、pH、电压及温度等参数对寡糖分离的影响。实验结果表明在pH=2.5,100mmol/L磷酸盐缓冲液中,运行电压20kV,电泳由负极到正极,温度30℃,235nm紫外检测条件下,酶解褐藻胶寡糖获得了高效分离。
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[外文摘要]
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A method for the
separation of alginate oligomers degraded by the
alginate myase with High Performance Capillary
Electrophoresis(HPCE) is presented.Brief
optimization studies have also been performed with respect to CE separation
conditions of oligomers.The oligomers
were analyzed in conditions of pH 2.5,100mmol/L phosphate buffer,-20kV,temperature
30℃,and 235nm UV detection.In this condition the alginate oligomers were successfully mapped.
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