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[篇名]
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羊栖菜岩藻聚糖硫酸酯的提取纯化和化学组成研究
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[著者]
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徐杰; 吕志华
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[机构]
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中国海洋大学食品科学与工程学院; 中国海洋大学医药学院， 山东 青岛 266003
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[中文关键词]
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羊栖菜; 岩藻聚糖硫酸酯; 提取; 纯化; 化学组成
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[外文关键词]
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Sargassum fusiforme; fucoidan; extraction; purification; chemical
composition
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[分类号]
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Q503
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[刊名]
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中国海洋大学学报
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[出版时间]
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2006年3月
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[来源]
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VOL36,NO.2._269—272
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[中文摘要]
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研究分析羊栖菜岩藻聚糖疏酸酯化学组成,为产品研制开发提供理论依据。采用稀酸提取和乙醇沉淀的方法得到羊栖菜岩藻聚糖硫酸酯粗品,粗品通过Q-Sepharose F.F.阴离子交换树脂进行分级纯化,得到硫酸基含量不同的3个组分(F-1,F-2和F-3),并对这3个组分的总糖、硫酸基、糖醛酸、氨基糖的含量及中性单糖组成和得率等进行了全面的分析。结果表明,羊栖菜岩藻聚糖硫酸酯主要由岩藻糖、半乳糖、甘露糖、葡萄糖及少量的鼠李糖、木糖和阿拉伯糖组成。3个组分中F-3的硫酸基含量最高(40.2%)但得率太低(0.8%);F-1的总糖(68.4%)和糖醛酸(17.9%)含量最高;3者氨基糖的含量均很低(≤1.0%);F-1,F-2和F-3相对分子质量分别约为210 000,160 000和140 000,F-2和F-3的纯度较高。
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[外文摘要]
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Crude fucoidans
were extracted with acidic solution,precipitated
with ethanol from Sargassum fusiforme,fractionalized
and purified into three fractions(F-1,F-2,F-3) with different sulfate ester
contents by anion-exchange chromatography on Q-Sepharose
F.F..The contents of total sugar,sulfate ester,uronic
acid and amino sugar,netural monosaccharide
composition and their yields were analyzed.The
results indicated that fucoidans from Sargassum fusiforme were
composed mainly of fucose,galactose,glctose,mannose
a...
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